Development of Films, Based on Oxidized Ipomea Batatas Starch, with Protein Encapsulation


  • José Alvarez-Barreto Universidad San Francisco de Quito, Ecuador
  • Daniela Viteri Narvaez Universidad San Francisco de Quito, Ecuador
  • Juan Sebastian Proaño Aviles Universidad San Francisco de Quito, Ecuador
  • Andrés Bernando Caicedo Páliz Universidad San Francisco de Quito, Ecuador
  • Michelle Amanda Grunauer Andrade Universidad San Francisco de Quito, Ecuador
  • Luis Ricardo Eguiguren Universidad San Francisco de Quito, Ecuador
  • Michel Vargas Escuela Politécnica Nacional, Ecuador



Scaffolds, Oxidation, Starch, Biomaterials, Biomedical applications


Oxidized or dialdehyde starches (DAS) have been used as biomaterials due to their biocompatibility and biodegradability; nonetheless, sweet potato (Ipomea batatas L.) starch has not been researched in this field. Films based on sweet potato DAS, mixed with native starch (NS), poly-vinyl alcohol (PVA) and glycerin have been developed to encapsulate a model protein (Bovine serum albumin, BSA), using central composite design (CCD) and surface methodology (RSM). Input variables were oxidation degree, NS concentration and polymeric mixture volume, while output variables were film´s thickness, equilibrium swelling and BSA release. DAS was obtained through hydrogen peroxide (H2O2) oxidation, and the oxidation degree is referred to as H2O2 concentration. Scanning electron microscopy revealed a rough surface, and formulations containing 10% H2O2 DAS presented micropores. Water uptake was greater with DAS than native starch, due to its hydroxyl groups shown in Fourier transformed infrared spectra. Fil thickness depended on the volume of the polymeric suspension and influenced the swelling capacity; thicker films absorbed less water. According to RSM, the optimal formulation was DAS with 5% H2O2 and 35% NS. Oxidized sweet potato starch has potential for biomaterial applications, as films developed with it can encapsulate a protein and release it in a controlled fashion.


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Chaulagain B, Jain A, Tiwari A, Verma A, et al. Passive delivery of protein drugs through transdermal route. Artif Cells Nanomed Biotechnol [Internet]. 2018;46(sup1):472–87. Available from:

López Angulo DE, do Amaral Sobral PJ. Characterization of gelatin/chitosan scaffold blended with aloe vera and snail mucus for biomedical purpose. Int J Biol Macromol [Internet]. 2016;92:645–53. Available from:

Sharma P, Kumar P, Sharma R, Bhatt VD, et al. Tissue Engineering; Current Status & Futuristic Scope. J Med Life [Internet]. 2019;12(3):225–9. Available from:

Augustine R, Hasan A, Dalvi YB, Rehman U, et al. Growth factor loaded in situ photocrosslinkable poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/gelatin methacryloyl hybrid patch for diabetic wound healing. Mater Sci Eng C [Internet]. 2021;118:111519. Available from:

Wu D, Bäckström E, Hakkarainen M. Starch Derived Nanosized Graphene Oxide Functionalized Bioactive Porous Starch Scaffolds. Macromol Biosci [Internet]. 2017;17(6):1600397. Available from:

Unnithan AR, Pichiah T, Gnanasekaran G, Seenivasan K, et al. Emu oil-based electrospun nanofibrous scaffolds for wound skin tissue engineering. Colloid Surf A-Physicochem Eng Asp [Internet]. 2012;415:454–60. Available from:

Panawes S, Ekabutr P, Niamlang P, Pavasant P, et al. Antimicrobial mangosteen extract infused alginate-coated gauze wound dressing. J Drug Deliv Sci Technol [Internet]. 2017;41:182–90. Available from:

Croisier F, Jérôme C. Chitosan-based biomaterials for tissue engineering. Eur Polym J [Internet]. 2013;49(4):780–92. Available from:

Wang J, Sun B, Tian L, He X, et al. Evaluation of the potential of rhTGF- β3 encapsulated P(LLA-CL)/collagen nanofibers for tracheal cartilage regeneration using mesenchymal stems cells derived from Wharton’s jelly of human umbilical cord. Mater Sci Eng C [Internet]. 2017;70(1):637–45. Available from:

Devi N, Sarmah M, Khatun B, Maji TK. Encapsulation of active ingredients in polysaccharide–protein complex coacervates. Adv Colloid Interface Sci [Internet]. 2017;239:136–45. Available from:

Shariatinia Z. Pharmaceutical applications of chitosan. Adv Colloid Interface Sci [Internet]. 2019;263:131–94. Available from:

Dhamecha D, Movsas R, Sano U, Menon JU. Applications of alginate microspheres in therapeutics delivery and cell culture: Past, present and future. Int J Pharm [Internet]. 2019;569:118627. Available from:

Ramanathan G, Singaravelu S, Muthukumar T, Thyagarajan S, et al. Design and characterization of 3D hybrid collagen matrixes as a dermal substitute in skin tissue engineering. Mater Sci Eng C [Internet]. 2017;72:359–70. Available from:

Van Nieuwenhove I, Salamon A, Peters K, Graulus G-J, et al. Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating. Carbohydr Polym [Internet]. 2016;152:129–39. Available from:

Capanema NSV, Mansur AAP, de Jesus AC, Carvalho SM, et al. Superabsorbent crosslinked carboxymethyl cellulose-PEG hydrogels for potential wound dressing applications. Int J Biol Macromol [Internet]. 2018;106:1218–34. Available from:

Yadav I, Rathnam VSS, Yogalakshmi Y, Chakraborty S, et al. Synthesis and characterization of polyvinyl alcohol- carboxymethyl tamarind gum based composite films. Carbohydr Polym [Internet]. 2017;165:159–68. Available from:

Li Y, Tan Y, Xu K, Lu C, et al. A biodegradable starch hydrogel synthesized via thiol-ene click chemistry. Polym Degrad Stab [Internet]. 2017;137:75–82. Available from:

Nasri-Nasrabadi B, Mehrasa M, Rafienia M, Bonakdar S, Behzad T, et al. Porous starch/cellulose nanofibers composite prepared by salt leaching technique for tissue engineering. Carbohydr Polym [Internet]. 2014;108:232–8. Available from:

Waghmare VS, Wadke PR, Dyawanapelly S, Deshpande A, et al. Starch based nanofibrous scaffolds for wound healing applications. Bioact Mater [Internet]. 2018;3(3):255-266. Available from:

Kalia S, Avérous L. Biodegradable and Biobased Polymers for Environmental and Biomedical Applications. Beverly, MA: Wiley; 2016. 501p.

Zuo Y, Liu W, Xiao J, Zhao X, et al. Preparation and characterization of dialdehyde starch by one-step acid hydrolysis and oxidation. Int J Biol Macromol [Internet]. 2017;103:1257–64. Available from:

Zhang Y-R, Wang X-L, Zhao G-M, Wang Y-Z. Preparation and properties of oxidized starch with high degree of oxidation. Carbohydr Polym [Internet]. 2012;87:2554–62. Available from:

Zhang S-D, Zhang Y-R, Wang X-L, Wang Y-Z. High Carbonyl Content Oxidized Starch Prepared by Hydrogen Peroxide and Its Thermoplastic Application. Staerke [Internet]. 2009;61:646–55. Available from:

Wen N, Lü S, Xu X, Ning P, et al. A polysaccharide-based micelle-hydrogel synergistic therapy system for diabetes and vascular diabetes complications treatment. Mater Sci Eng C [Internet]. 2019;100:94–103. Available from:

Nada AA, Soliman AAF, Aly AA, Abou-Okeil A. Stimuli-Free and Biocompatible Hydrogel via Hydrazone Chemistry: Synthesis, Characterization, and Bioassessment. Staerke [Internet]. 2018;71:1800243. Available from:

El Sheikha AF, Ray RC. Potential impacts of bioprocessing of sweet potato: Review. Crit Rev Food Sci Nutr [Internet]. 2017;57(3):455–71. Available from:

Smith RJ. Characterization and analysis of starches. In: Whistler RL, Paschall EF (eds.). Starch: Chemistry and Technology. New York: Academic Press; 1967:620–625p.

Parovuori P, Hamunen A, Forssell P, Autio K, et al. Oxidation of Potato Starch by Hydrogen Peroxide. Staerke [Internet]. 1995;47(1):19-23. Available from:

Ellens CJ. Design, optimization and evaluation of a free-fall biomass fast pyrolysis reactor and its products. [Master's thesis]. [Iowa]: Iowa State University, 2009: 155.

Nomani A, Nosrati H, Manjili HK, Khesalpour L, et al. Preparation and Characterization of Copolymeric Polymersomes for Protein Delivery. Drug Res [Internet]. 2017;67(8):458–65. Available from:

Osundahunsi OF, Fagbemi TN, Kesselman E, Shimoni E. Comparison of the Physicochemical Properties and Pasting Characteristics of Flour and Starch from Red and White Sweet Potato Cultivars. J Agric Food Chem [Internet]. 2003;51(8):2232–6. Available from:

Pérez S, Baldwin PM, Gallant DJ. Structural Featurs of Starch Granules I. In: BeMiller J, Whistler R (eds). Food Science and Technology [Internet]. Academic Press; 2009. 149-192p. Available from:

Salmi T, Tolvanen P, Wärnå J, Mäki-Arvela P, et al. Mathematical modeling of starch oxidation by hydrogen peroxide in the presence of an iron catalyst complex. Chem Eng Sci [Internet]. 2016;146:19–25. Available from:

Lyu Y, Ren H, Yu M, Li X, et al. Using oxidized amylose as carrier of linalool for the development of antibacterial wound dressing. Carbohydr Polym [Internet]. 2017;174:1095–105. Available from:

Silva R, Singh R, Sarker B, Papageorgiou DG, et al. Hydrogel matrices based on elastin and alginate for tissue engineering applications. Int J Biol Macromol [Internet]. 2018;114:614–25. Available from:

Nourmohammadi J, Ghaee A, Liavali SH. Preparation and characterization of bioactive composite scaffolds from polycaprolactone nanofibers-chitosan-oxidized starch for bone regeneration. Carbohydr Polym [Internet]. 2016;138:172–9. Available from:

Tang IM, Krishnamra N, Charoenphandhu N, Hoonsawat R, et al. Biomagnetic of Apatite-Coated Cobalt Ferrite: A Core–Shell Particle for Protein Adsorption and pH-Controlled Release. Nanoscale Res Lett [Internet]. 2011;6:19. Available from:

Li D, Ye Y, Li D, Li X, et al. Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin–PEG composite hydrogel fibers for wound dressings. Carbohydr Polym [Internet]. 2016;137:508–14. Available from:

Arockianathan PM, Sekar S, Kumaran B, Sastry TP. Preparation, characterization and evaluation of biocomposite films containing chitosan and sago starch impregnated with silver nanoparticles. Int J Biol Macromol [Internet]. 2012;50(4):939–46. Available from:

Moreno O, Cárdenas J, Atarés L, Chiralt A. Influence of starch oxidation on the functionality of starch-gelatin based active films. Carbohydr Polym [Internet]. 2017;178:147–58. Available from:

Liu Y, Ma L, Gao C. Facile fabrication of the glutaraldehyde cross-linked collagen / chitosan porous scaffold for skin tissue engineering. Mater Sci Eng C [Internet]. 2012;32(8):2361–6. Available from:

Li T, Song X, Weng C, Wang X, et al. Self-crosslinking and injectable chondroitin sulfate/pullulan hydrogel for cartilage tissue engineering. Appl Mater Today [Internet]. 2018;10:173–83. Available from:

Zareidoost A, Yousefpour M, Ghaseme B, Amanzadeh A. The relationship of surface roughness and cell response of chemical surface modification of titanium. J Mater Sci Mater Med [Internet]. 2012;23:1479–88. Available from:

Hou Y, Xie W, Yu L, Cuellar Camacho L, et al. Surface Roughness Gradients Reveal Topography-Specific Mechanosensitive Responses in Human Mesenchymal Stem Cells. Small[Internet].2020;16(10):1905422. Available from:




How to Cite

Alvarez-Barreto, J., Viteri Narvaez, D., Proaño Aviles, J. S., Caicedo Páliz, A. B., Grunauer Andrade, M. A., Eguiguren, L. R., & Vargas, M. . (2021). Development of Films, Based on Oxidized Ipomea Batatas Starch, with Protein Encapsulation. Revista Mexicana De Ingenieria Biomedica, 42(2), 119–131.



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