Modificación de electrodos-impresos con Azul de Prusia y enzima para el control de diabetes en sudor: Estudio preliminar de viabilidad: Modification of screen-printed electrodes with Prussian Blue (PB) and enzyme for diabetes control in sweat: Preliminary feasibility study

Mara Beltrán-Gastélum; Reyna Gutiérrez-Gutiérrez; Bruno U. Godínez-Dávalos; Moisés Israel Salazar-Gastélum; José Ricardo Cárdenas-Valdez; Sergio Pérez-Sicairos

doi:10.17488/RMIB.47.SI-TAIH.1521

Autores/as

Mara Beltrán-Gastélum Tecnológico Nacional de México/Instituto Tecnológico de Tijuana/Centro de Graduados e Investigación en Química https://orcid.org/0000-0002-3289-0664
Reyna Gutiérrez-Gutiérrez Tecnológico Nacional de México/Instituto Tecnológico de Tijuana/Posgrado en Ciencias de la Ingeniería.
Bruno U. Godínez-Dávalos Tecnológico Nacional de México/Instituto Tecnológico de Tijuana/Posgrado en Ciencias de la Ingeniería
Moisés Israel Salazar-Gastélum 1Tecnológico Nacional de México/Instituto Tecnológico de Tijuana/Posgrado en Ciencias de la Ingeniería. 2Tecnológico Nacional de México/Instituto Tecnológico de Tijuana/Centro de Graduados e Investigación en Química https://orcid.org/0009-0001-2996-8010
José Ricardo Cárdenas-Valdez Tecnológico Nacional de México/Instituto Tecnológico de Tijuana/Posgrado en Ciencias de la Ingeniería. https://orcid.org/0000-0002-5437-8215
Sergio Pérez-Sicairos Tecnológico Nacional de México/Instituto Tecnológico de Tijuana/Centro de Graduados e Investigación en Química

DOI:

https://doi.org/10.17488/RMIB.47.SI-TAIH.1521

Palabras clave:

Azul de Prusia, Electrodos impresos, Glucosa, Monitoreo no invasivo, Sensor enzimático

Resumen

La prevalencia de diabetes en México ha aumentado considerablemente, incrementando la necesidad de desarrollar nuevas plataformas de monitoreo de glucosa no invasivas y confiables. Esta investigación tiene como objetivo el desarrollo y evaluación de una plataforma de monitoreo no invasiva para la cuantificación de glucosa en el sudor. Dicha plataforma se basa en el uso de electrodos impresos modificados con un mediador electroquímico como el Azul de Prusia (PB) y con una enzima altamente específica, como la glucosa-oxidasa (GO_x). Adicionalmente, se proporciona el diseño de un dispositivo electrónico portátil para la generación de la señal de perturbación y el procesamiento de las señales de respuesta del analito. El sistema se evaluó en una solución buffer de fosfato salino (PBS) 0.1 M, con distintas concentraciones de glucosa en el intervalo esperado en el sudor. Los resultados demostraron que el PB desempeña un papel fundamental en la detección de glucosa, ya que el electrodo modificado únicamente con la enzima no muestra tendencia, mientras que el electrodo modificado con PB y enzima presentó un buen coeficiente de correlación, con una sensibilidad analítica de 0.0008 mA µM^-1. Este avance podría revolucionar el monitoreo de glucosa, facilitando tecnologías de salud más accesibles y menos invasivas.

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