Three-dimensional printing in healthcare

  • Alberto Isaac Pérez Sanpablo
  • Elisa Romero Avila RWTH Aachen
  • Arturo González Mendoza
Keywords: 3D printing, Stereolithography, Medicine


This work aims to briefly present the cutting edge of 3D printing innovation in healthcare. This technology is used for surgical planning, medical education, bioprinting of tissues, and medical equipment spare parts in fields like pharmacology, prosthetics, surgery, and regenerative medicine. A review of the last decade was made in the search engines of PubMed and Espacenet. Three authors reviewed titles, abstracts, and keywords separately to identify studies appropriate to the topic. After the initial examination, complete texts of identified relevant studies were obtained and classified according to the authors. Results were synthesized in a narrative literature review. The revision showed that 3D printing has become of common use in the healthcare system since it allows medical personnel to implement customized solutions for each patient, thus reducing the probability of a false diagnostic or treatment. Major applications among the advantages and disadvantages of 3D printing in healthcare were presented. Nowadays, the main challenge in 3D printing is the cost of the equipment and its manufacturing. In the future, the challenges in cost could be reduced, but processing requirements and limited materials may still need further work.


Hurst EJ. 3D Printing in Healthcare: Emerging Applications. J Hosp Librariansh [Internet]. 2016;16(3):255–67. Available from:

Mukhopadhyay S, Poojary R. A review on 3D printing: Advancement in healthcare technology. In 2018 Advances in Science and Engineering Technology International Conferences (ASET) [Internet]. Abu Dhabi: IEEE; 2018:1-5. Available from:

Mishra S. Application of 3D printing in medicine. Indian Heart J [Internet]. 2016;68(1):108–109. Available from:

Ozbolat IT, Peng W, Ozbolat V. Application areas of 3D bioprinting. Drug Discov Today [Internet]. 2016;21(8):1257–1271. Available from:

Chen JKC, Do HTT. Perspective of the 3D Printing Technology Applied on Medical Resource Integration and Service Innovation Business Model. In 2017 Portland International Conference on Management of Engineering and Technology (PICMET) [Internet]. Portland: IEEE; 2017:1–11. Available from:

Langridge B, Momin S, Coumbe B, et al. Systematic Review of the Use of 3-Dimensional Printing in Surgical Teaching and Assessment. J Surg Educ [Internet]. 2018;75(1):209–221. Available from:

Vaillant T, Steelandt J, Cordonnier A-L, et al. Revue des guides personnalisés à usage unique dans les prothèses totales de genou. Ann Pharm Fr [Internet]. 2018;76(3):228-234. Available from:

Nagarajan NN, Dupret-Bories A, Karabulut E, et al. Enabling personalized implant and controllable biosystem development through 3D printing. Biotechnol Adv [Internet]. 2018;36(2):521–533. Available from:

Tan XP, Tan YJ, Chow CSL, et al. Metallic powder-bed based 3D printing of cellular scaffolds for orthopaedic implants: A state-of-the-art review on manufacturing, topological design, mechanical properties and biocompatibility. Mater Sci Eng C Mater Biol Appl [Internet]. 2017;76(1):1328–1343. Available from:

Savonen B, Gershenson J, Bow JK, et al. Open-Source Three-Dimensional Printable Infant Clubfoot Brace. JPO J Prosthetics Orthot [Internet]. 2020;32(2):149–158. Available from:

de Souza MA, Schmitz C, Pinhel MM, et al. Proposal of custom made wrist orthoses based on 3D modelling and 3D printing. In 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) [Internet]. Jeju: IEEE; 2017:3789–3792. Available from:

Awad A, Trenfield SJ, Gaisford S, et al. 3D printed medicines: A new branch of digital healthcare. Int J Pharm [Internet]. 2018;548(1):586–596. Available from:

Trenfield SJ, Awad A, Goyanes A, et al. 3D Printing Pharmaceuticals: Drug Development to Frontline Care. Trends Pharmacol Sci [Internet]. 2018;39(5):440–451. Available from:

Xia R-Z, Zhai Z-J, Chang Y-Y, Li H-W. Clinical Applications of 3-Dimensional Printing Technology in Hip Joint. Orthop Surg [Internet]. 2019;11(4):533–544. Available from:

Liang K, Brambilla D, Leroux J-C. Is 3D Printing of Pharmaceuticals a Disruptor or Enabler? Adv Mater [Internet]. 2019;31(5):e1805680. Available from:

Ong CS, Yesantharao P, Huang CY, et al. 3D bioprinting using stem cells. Pediatr Res [Internet]. 2018;83:223–231. Available from:

VanKoevering KK, Malloy KM. Emerging Role of Three-Dimensional Printing in Simulation in Otolaryngology. Otolaryngol Clin North Am [Internet]. 2017;50(5):947–958. Available from:

Huang Y, Zhang X-F, Gao G, et al. 3D bioprinting and the current applications in tissue engineering. Biotechnol J [Internet]. 2017;12(8):1600734. Available from:

Lee VK, Dai G. Printing of Three-Dimensional Tissue Analogs for Regenerative Medicine. Ann Biomed Eng [Internet]. 2017;45:115–131. Available from:

Sandler N, Preis M. Printed Drug-Delivery Systems for Improved Patient Treatment. Trends Pharmacol Sci [Internet]. 2016;37(12):1070–1080. Available from:

Shahar FS, Hameed Sultan MT, Lee SH, et al. A review on the orthotics and prosthetics and the potential of kenaf composites as alternative materials for ankle-foot orthosis. J Mech Behav Biomed Mater [Internet]. 2019;99:169–185. Available from:

Hooper J, Schwarzkopf R, Fernandez E, et al. Feasibility of single-use 3D-printed instruments for total knee arthroplasty. Bone Joint J [Internet]. 2019;101-B(7-C):115–120. Available from:

Tang X, Qin Y, Xu X, et al. Fabrication and In Vitro Evaluation of 3D Printed Porous Polyetherimide Scaffolds for Bone Tissue Engineering. Biomed Res Int [Internet]. 2019; 2019:2076138. Available from:

Theodoridis K, Aggelidou E, Manthou M, et al. Assessment of cartilage regeneration on 3D collagen-polycaprolactone scaffolds: Evaluation of growth media in static and in perfusion bioreactor dynamic culture. Colloids Surf B Biointerfaces [Internet]. 2019;183(1):110403. Available from:

Wu Y-HA, Chiu Y-C, Lin Y-H, et al. 3D-Printed Bioactive Calcium Silicate/Poly-ε-Caprolactone Bioscaffolds Modified with Biomimetic Extracellular Matrices for Bone Regeneration. Int J Mol Sci [Internet].2019;20(4):942. Available from:

Stanislavovna AO, Mironovich BS, Valerevich SS, et al., inventors; Method of producing ceramic sample based on β-tricalcium phosphate using stereolithography technique for recovering bone tissue [Internet]. RU2729761C1. 2020. Available from:

Sergeevich AA, Darchoevich AS, Borisovna AN, et al., inventors; Method of facial prosthesis manufacturing [Internet]. RU2727741C1. 2020. Available from:

Yubo F, Wanru F, Xiaoming L, et al., inventors; Multi-arm cooperation type biological three-dimensional printing device [Internet]. CN110435144A. 2019. Available from:

Yubo F, Wenyong L, Baosen T, inventors; Intelligent multisource synchronous orienting three-dimensional printing device [Internet]. CN108891025A. 2018. Available from:

Ayberk Y, invertor; Implant guide system [Internet]. WO2009004526A2. 2009. Available from:

Vladimirovich PJ, Akhmadovich SS, Evich SAE, invertors; Method for nail osteosynthesis of facial bone fractures [Internet]. RU2523828C1. 2014. Available from:

Vladimirovich PJ, Evich SAE, inventors; Method for surgical approach to periapical tissues of jaw [Internet]. RU2523352C1. 2014. Available from:

Rui G, Yuyang L, Xiaodong L, et al., inventors; Orbital blow-out fracture titanium mesh prefabrication method [Internet]. CN107374785A. 2018. Available from:

Sergeevich BS, Andreevich CA, Olegovich da, et al., inventors; Method for replacement of defects of proximal tibia when performing knee joint endoprosthesis replacement and device for its implementation [Internet]. RU2730985C1. 2020. Available from:

Choi Y-J, Yi H-G, Kim S-W, et al. 3D Cell Printed Tissue Analogues: A New Platform for Theranostics. Theranostics [Internet]. 2017;7(12):3118–3137. Available from:

Skeldon G, Lucendo-Villarin B, Shu W. Three-dimensional bioprinting of stem-cell derived tissues for human regenerative medicine. Philos Trans R Soc Lond B Biol Sc i [Internet]. 2018;373(1750): 20170224. Available from:

Yujian K, Xiao Z, inventors; Biobrick used for biological printing and application thereof [Internet]. CN106039419A. 2016. Available from:

James KY, Xiao Z, inventors; Compositions for cell-based three dimensional printing [Internet]. WO2016161944A1. 2016. Available from:

Yujian K, Xiao Z, inventors; Method for preparing constructed body from biobrick containing endothelial cells [Internet]. CN106039409A. 2016. Available from:

Yuyu H, Shuangshuang M, Wei S, et al., inventors; Frozen gel three-dimensional structural body, preparation method and application thereof [Internet]. CN106178110A. 2016. Available from:

Haiqin D, Liumin H, Huiheng L, et al., inventors; Preparation and application of linear biodegradable polyester elastomer with controllable elasticity and shape memory effect [Internet]. CN105504248A. 2016. Available from

Yuxiong G, Pan J, Xiaolong W, et al., inventors; 3D (three-dimensional) printing biomedical hydrogel and method for preparing same [Internet]. CN108424533A. 2018. Available from:

Zhi H, Lin M, Qing T, et al., inventors; Photo-thermal chemotherapy bone repair material and preparation method of tissue engineering scaffold [Internet]. CN109010925A. 2018. Available from:

Qianqian D, Jianlong J, Aoqun J, et al., inventors; Biological 3D printed full-custom skin and preparation method thereof [Internet]. CN108392676A. 2018. Available from:

Ming’en X, Rui Y, inventors; Pancreatic-like structural body and construction method and application thereof [Internet]. CN111197024A. 2020. Available from:

Domogatskij, Osidak, inventors; Sterile transparent concentrated solution of biocompatible collagen, method for production and use thereof [Internet]. RU2715715C1. 2020. Available from:

Jintao C, Taiying C, Kang L, inventors; 3D printing bone repairing bracket with antibacterial property and preparation method of 3D printing bone repairing bracket [Internet]. CN106729988A. 2017. Available from:

Jun AM, Woo CD, Jin CY, et al., inventors; 3 Wet 3D cell printing using decellularized extracellular matrix [Internet]. KR20180049712A. 2018. Available from:

Peng P, Baoqing Y, Xu Z, et al., inventors; Three-dimensional printing strontium-containing mesoporous bioglass bracket loaded with soy isoflavone and preparation method thereof [Internet]. CN106267375. 2017. Available from:

Yujiang F, Xuan P, Yaning W, et al., inventors; Bionics design bone-line porous bone product and preparation method and purpose thereof [Internet]. CN105877874A. 2016. Available from:

Qi L, Rong W, Weiyi Y, et al., inventors; Three-dimensional biological scaffold, preparation method and applications thereof [Internet]. CN109381739A. 2019. Available from:

Brown A, Chester D, Daniele M, et al., inventor; Three-dimensional printing of colloidal building blocks for wound healing materials [Internet]. WO2019195681A1. 2019. Available from:

Wenrui C, Cuihai D, Wenbo H, et al., inventors; Multi-pore bionic skull repair material and individualized manufacturing method [Internet]. CN108273137A. 2018. Available from:

Chunyan B, Yujie H, Qiuning L, et al., Preparation, raw material, product and application of photo-coupling co-crosslinking hydrogel material [Internet]. CN109776450A. 2019. Available from:

Yingfang A, Guocheng D, Xiaoqing H, et al., inventors; Preparation method of cell 3D printing bio-ink with good printability [Internet]. CN111544657A. 2020. Available from:

Moroni L, Boland T, Burdick JA, et al. Biofabrication: A Guide to Technology and Terminology. Trends Biotechnol [Internet]. 2018 36(4):384–402. Available from:

Aljohani W, Ullah MW, Zhang X, et al. Bioprinting and its applications in tissue engineering and regenerative medicine. Int J Biol Macromol [Internet]. 2018;107(Part A): 261–275. Available from:

Zhang YS, Oklu R, Dokmeci MR, et al. Three-Dimensional Bioprinting Strategies for Tissue Engineering. Cold Spring Harb Perspect Med [Internet]. 2017;8(2):a025718. Available from:

Palo M, Holländer J, Suominen J, et al. 3D printed drug delivery devices: perspectives and technical challenges. Expert Rev Med Devices [Internet]. 2017;14(9):685–696. Available from:

Preis M, Oblom H. 3D-Printed Drugs for Children-Are We Ready Yet? AAPS PharmSciTech [Internet]. 2017;18:303–308. Available from:

Kaae S, Lind JLM, Genina N, et al. Unintended consequences for patients of future personalized pharmacoprinting. Int J Clin Pharm [Internet]. 2018;40:321–324. Available from:

Randazzo M, Pisapia JM, Singh N, et al. 3D printing in neurosurgery: A systematic review. Surg Neurol Int [Internet]. 2016;7(S33):S801–S809. Available from:

Shah KJ, Peterson JC, Chamoun R. 3D Printed Models in Neurosurgical Training BT - Comprehensive Healthcare Simulation: Neurosurgery. In: Alaraj A (ed). Comprehensive Healthcare Simulation: Neurosurgery. Comprehensive Healthcare Simulation [Internet]. Cham: Springer International Publishing; 2018: 47–64. Available from:

Vakharia VN, Vakharia NN, Hill CS. Review of 3-Dimensional Printing on Cranial Neurosurgery Simulation Training. World Neurosurg [Internet]. 2016;88:188–198. Available from:

McMillan A, Kocharyan A, Dekker SE, et al. Comparison of Materials Used for 3D-Printing Temporal Bone Models to Simulate Surgical Dissection. Ann Otol Rhinol Laryngol [Internet]. 2020;129(12):1168–1173. Available from:

Wu A, inventor; Single-action three-dimensional model printing methods [Internet]. US8579620B2. 2011. Available from:

Pucci JU, Christophe BR, Sisti JA, et al. Three-dimensional printing: technologies, applications, and limitations in neurosurgery. Biotechnol Adv [Internet]. 2017;35(5):521–529. Available from:

Boland T, Wilson JWC, Xu T, inventors; Ink-jet printing of viable cells [Internet]. US7051654B2. 2003. Available from:

Kang H-W, Lee SJ, Atala A, Yoo JJ, inventors; Integrated organ and tissue printing methods, system and apparatus [Internet]. US20120089238A1. 2010. Available from:

Wu Y, Vida VL, Zheng M, Yang J. Progress and Prospects of Cardiovascular 3D Printing BT - Cardiovascular 3D Printing: Techniques and Clinical Application. In: Yang J, Lee AP-W, Vida VL (eds). Cardiovascular 3D Printing [Internet]. Singapore: Springer Singapore; 2021: 179–185. Available from:

Zhang YS, Yue K, Aleman J, et al. 3D Bioprinting for Tissue and Organ Fabrication. Ann Biomed Eng [Internet]. 2017;45:148–163. Available from:

Ferrari E, Gallo M, Wang C, et al. Three-dimensional printing in adult cardiovascular medicine for surgical and transcatheter procedural planning, teaching and technological innovation. Interact Cardiovasc Thorac Surg [Internet]. 2020;30(2):203–214. Available from:

Xu C, Liang J, Yang J. History of Cardiovascular 3D Printing BT - Cardiovascular 3D Printing: Techniques and Clinical Application. In: Yang J, Lee AP-W, Vida VL (eds) Cardiovascular 3D Printing [Internet]. Singapore: Springer Singapore; 2021: 1–2. Available from:

Valverde I. Three-dimensional Printed Cardiac Models: Applications in the Field of Medical Education, Cardiovascular Surgery, and Structural Heart Interventions. Rev Esp Cardiol (Engl Ed) [Internet]. 2017;70(4):282–291. Available from:

Baribeau Y, Sharkey A, Mahmood E, et al. Three-Dimensional Printing and Transesophageal Echocardiographic Imaging of Patient-Specific Mitral Valve Models in a Pulsatile Phantom Model. J Cardiothorac Vasc Anesth [Internet]. 2019;33(12):3469–3475. Available from:

Wang Z, Mithieux SM, Weiss AS. Fabrication Techniques for Vascular and Vascularized Tissue Engineering. Adv Healthc Mater [Internet]. 2019;8(19):1900742. Available from:

Mok S-W, Nizak R, Fu S-C, et al. From the printer: Potential of three-dimensional printing for orthopaedic applications. J Orthop Transl [Internet]. 2016;6:42–49. Available from:

Wang Y, Gao M, Wang D, et al. Nanoscale 3D Bioprinting for Osseous Tissue Manufacturing. Int J Nanomedicine [Internet]. 2020;15:215–226. Available from:

Alkhouri N, Zein NN. Three-dimensional printing and pediatric liver disease. Curr Opin Pediatr [Internet]. 2016;28(5):626–630. Available from:

Wei S, Rui Y, inventors; Construction method and application of in-vitro three-dimensional human liver tissue [Internet]. CN106916781A. 2017. Available from:

Jintao C, Taiying C, Kang L, inventors; Skin repair material with biological activity and method for preparing skin repair material [Internet]. CN106421931A. 2017. Available from:

Arealis G, Nikolaou VS. Bone printing: new frontiers in the treatment of bone defects. Injury [Internet]. 2015;46(S8):S20-2. Available from:

Kaye R, Goldstein T, Zeltsman D, et al. Three dimensional printing: A review on the utility within medicine and otolaryngology. Int J Pediatr Otorhinolaryngol [Internet]. 2016;89:145–148. Available from:

Qingzi B, Yunqi B, Jingsong L, et al., inventors; 4D-printing shape-memory-polymer-composite-material tracheal stent and preparing method thereof [Internet]. CN108969165A. 2018. Available from:

Valerevich MS, Aleksandrovich PA, inventors; Method for manufacture of individual implant for skull bone defects replacement [Internet]. RU2644275C1. 2018. Available from:

Chen MY, Skewes J, Desselle M, et al. Current applications of three-dimensional printing in urology. BJU Int [Internet]. 2020;125(1):17–27. Available from:

Parikh N, Sharma P. Three-Dimensional Printing in Urology: History, Current Applications, and Future Directions. Urology [Internet]. 2018;121:3–10. Available from:

Childs BS, Manganiello MD, Korets R. Novel Education and Simulation Tools in Urologic Training. Curr Urol Rep [Internet]. 2019;20:81. Available from:

Tatar İ, Huri E, Selçuk İ, et al. Review of the effect of 3D medical printing and virtual reality on urology training with ‘MedTRain3DModsim’ Erasmus + European Union Project. Turkish J Med Sci [Internet]. 2019;49:1257–1270. Available from:

Severini C, Derossi A. Could the 3D Printing Technology be a Useful Strategy to Obtain Customized Nutrition? J Clin Gastroenterol [Internet]. 2016;50:S175–S178. Available from:

Chang D, Tummala S, Sotero D, et al. Three-Dimensional Printing for Procedure Rehearsal/Simulation/Planning in Interventional Radiology. Tech Vasc Interv Radiol [Internet]. 2019;22(1):14–20. Available from:

Hodgdon T, Danrad R, Patel MJ, et al. Logistics of Three-dimensional Printing: Primer for Radiologists. Acad Radiol [Internet]. 2018;25(1):40–51. Available from:

Mitsouras D, Liacouras P, Imanzadeh A, et al. Medical 3D Printing for the Radiologist. Radiographics [Internet]. 2015;35(7):1965–1988. Available from:

Martín Noguerol T, Paulano-Godino F, Martín-Valdivia MT, et al. Strengths, Weaknesses, Opportunities, and Threats Analysis of Artificial Intelligence and Machine Learning Applications in Radiology. J Am Coll Radiol [Internet]. 2019;16(9):1239–1247. Available from:

U.S. Food & Drug Administration. 3D Printing of Medical Devices. U.S. Food & Drug Administration [Internet]. 2019. Available from:

Stratasys Direct Manufacturing. Top Challenges to Widespread 3D Printing Adoption. MachineDesing [Internet]. 2019. Available from:

Abudayyeh I, Gordon B, Ansari MM, et al. A practical guide to cardiovascular 3D printing in clinical practice: Overview and examples. J Interv Cardiol [Internet]. 2018;31(3):375–383. Available from:

VanKoevering KK, Hollister SJ, Green GE. Advances in 3-Dimensional Printing in Otolaryngology: A Review. JAMA Otolaryngol Head Neck Surg [Internet]. 2017;143(2):178–183. Available from:

How to Cite
Pérez Sanpablo, A. I., Romero Avila, E., & González Mendoza, A. (2021). Three-dimensional printing in healthcare. Mexican Journal of Biomedical Engineering, 42(2), 32-48. Retrieved from
Review Article