Antibacterial Activity Analysis of Hydroxyapatite Based Materials with Fluorine and Silver

  • Verónica González Torres Facultad de Ciencias de la Salud Universidad Autónoma de Baja California
  • Esteban Hernández Guevara Facultad de Ciencias Químicas e Ingeniería Universidad Autónoma de Baja California
  • Nydia Alejandra Castillo Martínez Facultad de Ciencias de la Salud Universidad Autónoma de Baja California
  • Martha Rosales Aguilar Facultad de Medicina y Psicología Universidad Autónoma de Baja California
  • César Gerardo Díaz Trujillo
Keywords: Hydroxyapatite, Antibacterial, Silver


This investigation aims to evaluate the antibacterial activity of nanostructured hydroxyapatite based materials doped with silver and fluorine, to be used as a biomaterial with antibacterial activity. Four different formulations were prepared by combustion method: hydroxyapatite, hydroxyapatite-fluorine, hydroxyapatite-silver-fluorine and hydroxyapatite-silver, with 2% of the doping agents. X-ray diffraction technique was used to determine the mineralogy, identifying the presence of Ca5(PO4)3OH, Ca2P2O7, Ag3PO4, AgCa10(PO4)7 Ca5(PO4)3F and CaF2 phases for the studied samples. Scanning electron microscopy was used to study the morphological structure and it showed homogeneous crystallization of the hydroxyapatite and the inclusion of dopant agents. The antibacterial activity was determined using a modified inhibition test zone to observe if the bacteria (E. faecalis) was susceptible to the antimicrobial agent by the appearance of the zone of inhibition on the agar plate. Both the hydroxyapatite-silver and the hydroxyapatite-silver-fluorine materials generated an inhibition zone. It was possible to determine the minimum inhibitory concentration needed to kill most viable organisms after 48 hours of incubation using the broth microdilution method, resulting in 75 µg/ml and 200 µg/ml for the hydroxyapatite-silver and the hydroxyapatite-silver-fluorine formulation, respectively. These materials could be used for the development of new biomaterials that can be used in dental applications.

Author Biography

Verónica González Torres, Facultad de Ciencias de la Salud Universidad Autónoma de Baja California

Doctora en Ciencias de la Salud egresada de la Facultad de Odontología Tijuana de la Universidad Autónoma de Baja California.

Maestra en Ciencias con especialidad en Sistemas Ambientales egresada del Instituto Tecnológico y de Estudios Superiores de Monterrey (Campus Monterrey).

Químico Industrial egresada de la Facultad de Ciencia Químicas e Ingeniería de la Unviersidad Autónoma de Baja California.

Profesor de tiempo completo definitivo con nombramiento de profesor investigador adscrita a la Escuela de Ciencias de la Salud Valle de las Palmas de la Universidad Autónoma de Baja California.

Perfil deseable PRODEP.

Miembro del Cuerpo Académico en formación: Química Aplicada.

Docente de cátedra: bioquímica, estadística, epidemiología.

Cargo administrativo: Subdirectora 


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How to Cite
González Torres, V., Hernández Guevara, E., Castillo Martínez, N. A., Rosales Aguilar, M., & Díaz Trujillo, C. G. (2021). Antibacterial Activity Analysis of Hydroxyapatite Based Materials with Fluorine and Silver. Mexican Journal of Biomedical Engineering, 42(2), 49-57. Retrieved from
Research Articles