Cultivo de Células Madre Mesenquimales Sobre Hidrogeles Compuestos de Nanopartículas de Hidroxiapatita y Quitosano Foto-Entrecruzable

J. Alvarez-Barreto; K. Márquez; E. Gallardo; J. Moret; N. Benítez; M. Meyer; S. Marsiccobetre; D. Zujur

doi:10.17488/RMIB.38.3.2

Mesenchymal Stem Cell Culture on Composite Hydrogels of Hydroxyapatite Nanoparticles and Photo-Crosslinking Chitosan

Authors

J. Alvarez-Barreto Instituto de Energías y Materiales Alternativos, IDEMA. Departamento de Ingeniería Química. Universidad San Francisco de Quito. Quito, Ecuador
K. Márquez Laboratorio de Ingeniería de Tejidos Humanos. Fundación Instituto de Estudios Avanzados, IDEA. Caracas, Venezuela/Instituto de Biología Experimental. Universidad Central de Venezuela.
E. Gallardo Laboratorio de Ingeniería de Tejidos Humanos. Fundación Instituto de Estudios Avanzados, IDEA. Caracas, Venezuela
J. Moret Laboratorio de Ingeniería de Tejidos Humanos. Fundación Instituto de Estudios Avanzados, IDEA. Caracas, Venezuela
N. Benítez Laboratorio de Ingeniería de Tejidos Humanos. Fundación Instituto de Estudios Avanzados, IDEA. Caracas, Venezuela
M. Meyer Laboratorio de Ingeniería de Tejidos Humanos. Fundación Instituto de Estudios Avanzados, IDEA. Caracas, Venezuela
S. Marsiccobetre Laboratorio de Genómica y Proteómica. Fundación Instituto de Estudios Avanzados, IDEA. Caracas, Venezuela
D. Zujur Laboratorio de Ingeniería de Tejidos Humanos. Fundación Instituto de Estudios Avanzados, IDEA. Caracas, Venezuela/Departamento de Ciencias de los Materiales. Universidad Simón Bolívar. Valle de Sartenejas, Caracas, Venezuela

DOI:

https://doi.org/10.17488/RMIB.38.3.2

Keywords:

hydrogels, mesenchymal stem cells, hydroxyapatite, tissue engineering, photo-crosslinking

Abstract

Chitosan (QT) is a biopolymer that has been used in widely used in bone tissue engineering applications, demons-trating great potential for that purpose. Therefore, the present study aims to develop an in situ crosslinking hydro-gel system, composed of chitosan and hydroxyapatite (HAP). Briefly, chitosan was modified, using carbodiimide chemistry, with lactobionic and azidobenzoic acid to make it soluble at physiological pH and photo-crosslinkable, respectively. The modified chitosan was mixed with HAP, in different proportions, and later exposed to UV light, yielding hydrogels. Mesenchymal stem cells, from rat bone marrow, were seeded onto the hydrogels and cultured for 4, 10 and 16 days, under osteogenic and non-osteogenic conditions. Through cell proliferation and alkaline phosphatase activity assays, and confocal microscopy, it was observed that the majority of formulations supported cell adhesion and proliferation, and a significant early osteogenic differentiation in formulations 70:30 and 50:50, QT:HAP. According to these results, the proposed photo-crosslinking system has potential for tissue engineering applications, and further specific studies are proposed for cell differentiation.

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Published

2017-09-13

How to Cite

Alvarez-Barreto, J., Márquez, K., Gallardo, E., Moret, J., Benítez, N., Meyer, M., Marsiccobetre, S., & Zujur, D. (2017). Mesenchymal Stem Cell Culture on Composite Hydrogels of Hydroxyapatite Nanoparticles and Photo-Crosslinking Chitosan. Revista Mexicana De Ingenieria Biomedica, 38(3), 524–536. https://doi.org/10.17488/RMIB.38.3.2

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Issue

Vol. 38 No. 3 (2017)

Section

Research Articles

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