Development of Films, Based on Oxidized Ipomea Batatas Starch, with Protein Encapsulation

José Alvarez-Barreto; Daniela Viteri Narvaez; Juan Sebastian Proaño Aviles; Andrés Bernando Caicedo Páliz; Michelle Amanda Grunauer Andrade; Luis Ricardo Eguiguren; Michel  Vargas

doi:10.17488/RMIB.42.2.10

Authors

José Alvarez-Barreto Universidad San Francisco de Quito, Ecuador https://orcid.org/0000-0002-4019-4319
Daniela Viteri Narvaez Universidad San Francisco de Quito, Ecuador
Juan Sebastian Proaño Aviles Universidad San Francisco de Quito, Ecuador https://orcid.org/0000-0002-8609-2203
Andrés Bernando Caicedo Páliz Universidad San Francisco de Quito, Ecuador
Michelle Amanda Grunauer Andrade Universidad San Francisco de Quito, Ecuador
Luis Ricardo Eguiguren Universidad San Francisco de Quito, Ecuador
Michel Vargas Escuela Politécnica Nacional, Ecuador

DOI:

https://doi.org/10.17488/RMIB.42.2.10

Keywords:

Scaffolds, Oxidation, Starch, Biomaterials, Biomedical applications

Abstract

Oxidized or dialdehyde starches (DAS) have been used as biomaterials due to their biocompatibility and biodegradability; nonetheless, sweet potato (Ipomea batatas L.) starch has not been researched in this field. Films based on sweet potato DAS, mixed with native starch (NS), poly-vinyl alcohol (PVA) and glycerin have been developed to encapsulate a model protein (Bovine serum albumin, BSA), using central composite design (CCD) and surface methodology (RSM). Input variables were oxidation degree, NS concentration and polymeric mixture volume, while output variables were film´s thickness, equilibrium swelling and BSA release. DAS was obtained through hydrogen peroxide (H₂O₂) oxidation, and the oxidation degree is referred to as H₂O₂ concentration. Scanning electron microscopy revealed a rough surface, and formulations containing 10% H₂O₂ DAS presented micropores. Water uptake was greater with DAS than native starch, due to its hydroxyl groups shown in Fourier transformed infrared spectra. Fil thickness depended on the volume of the polymeric suspension and influenced the swelling capacity; thicker films absorbed less water. According to RSM, the optimal formulation was DAS with 5% H₂O₂ and 35% NS. Oxidized sweet potato starch has potential for biomaterial applications, as films developed with it can encapsulate a protein and release it in a controlled fashion.

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